• Product NameFZR1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total FZR1 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human FZR1
• Target NameFZR1
• Alternative Names    FZR; CDH1; FZR2; HCDH; HCDH1; CDC20C
• Accession No. Swiss-Prot#: Q9UM11
  Gene ID: 51343
• UniprotQ9UM11
• Gene ID51343;
• Calculated MW 55kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane: Human placenta tissue
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 3 minutes
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #43031 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43031 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting